single-component tmb substrate solution (Beijing Solarbio Science)
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single-component tmb substrate solution
Single Component Tmb Substrate Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+single-component+substrate+solution/tmb+single+component+substrate+solution/10__1016_slash_j__snb__2025__137923-61-0-7
Average 90 stars, based on 1 article reviews
Single Component Tmb Substrate Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+single-component+substrate+solution/tmb+single+component+substrate+solution/10__1016_slash_j__snb__2025__137923-61-0-7
Average 90 stars, based on 1 article reviews
single-component tmb substrate solution - by Bioz Stars,
2026-10
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Incubation:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Concentration Assay:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Indirect ELISA:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Enzyme-linked Immunosorbent Assay:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Virus:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Infection:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Recombinant:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Modification:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of Staining:Article Title: Lipid-coated nanoparticles enhance the delivery of bacterial virulence factors as a potent toxoid vaccine platform against bacterial infections. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ɑ-hemolysin primary antibodies Sigma-Aldrich Cat# S7531; RRID: AB_261550 Anti-CD45R monoclonal antibody ThermoFisher Cat# 12-0452-82; RRID: AB_465671 Anti-CD3 monoclonal antibody ThermoFisher Cat# 25-0032-82; RRID: AB_2815096 Anti-CD4 monoclonal antibody BD Biosciences Cat# 564419; RRID: AB_2744419 Anti-CD8a monoclonal antibody BD Biosciences Cat# 563332; RRID: AB_2721167 HRP-conjugated goat anti-mouse IgG secondary antibody ZSGB-Bio Cat# PV-6002; RRID: AB_2864334 HRP-conjugated affinipure goat anti-swine IgG (H + L) Proteintech Cat# SA00001-5; RRID: AB_2890883 Bacterial and virus strains Methicillin-resistant Staphylococcus aureus (USA300) ATCC Cat# BAA-1717 Clinical Staphylococcus aureus-1 This paper N/A Clinical Staphylococcus aureus-2 This paper N/A Clinical Staphylococcus aureus-3 This paper N/A Pseudomonas aeruginosa (PA-103) ATCC Cat# 29260 Biological samples Human blood samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human sputum samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Human skin samples from infected patient The second affiliated hospital of Wenzhou Medical University N/A Chemicals, peptides, and recombinant proteins Sphingomyelin Avanti Polar Lipids Cat# 860061P; CAS: 383907-87-7 Phosphatidylcholine AVT Cat# N01001; CAS: 93685-90-6 DSPE-mPEG2000 AVT Cat# F01008; CAS: 147867-65-0 Cholesterol AVT Cat# O01001; CAS: 57-88-5 Poly (lactic-co-glycolic acid) (PLGA) LACTEL Absorbable Polymers CAS# 26780-50-7 100× penicillin-streptomycin Meilunbio Cat# MA0110 Dulbecco’s Modified Eagle Medium Meilunbio Cat# PWL037 Fetal bovine serum Biological Industries Cat# 2140300 Hematoxylin and eosin (H&E) staining solution Solarbio Cat# Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling Article Snippet: The reaction was incubated with 100 μL of Article Title: Establishment of an indirect ELISA detection method for porcine reproductive and respiratory syndrome virus NSP4 Article Snippet: After another Article Title: Protection against lethal HAdV-4 challenge in STAT1 mice by novel human monoclonal antibodies Article Snippet: Following another wash with PBST, Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV. Article Snippet: Then, the plateswere incubated for 1 h andwashedwith PBST.The assaywas developed for 5min at RT in the dark using 100 μL of Article Title: Establishment of an indirect ELISA antibody detection method based on the stable expression of LSDV P32 protein in CHO-K1 cells. Article Snippet: Establishment of an indirect ELISA method based on P32 protein The original ELISA protocol is as follows: The P32 protein was diluted to 2 μg/mL using carbonate buffer (CBS, pH 9.6), and 100 μL/well was coated onto a 96-well microplate at 37 °C for 2 h. The plate was then blocked with 5% skimmed milk (200 μL/well) at 37 °C for 2 h. Subsequently, LSDV-positive and LSDV-negative sera, diluted at 1:400 (in PBS), were added (100 μL/well) and incubated at 37 °C for 2 h. The enzyme-labeled antibody (rabbit anti-bovine IgG-HRP, CUSABIO) was diluted at 1:5000 (in PBS), added (100 μL/well), and incubated at 37 °C for 1 h. Next, 100 μL of Article Title: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV Article Snippet: The assay was developed for 5 min at RT in the dark using 100 μL of Article Title: A new type of Caspase-1 upon recognizing bacteria inhibits GSDME-dependent histone modification and NF-κB signaling. Article Snippet: The reaction was incubated with 100 μL of |